|
KMID : 0895820070170040041
|
|
Journal of Oriental Rehabilitation Medicine
2007 Volume.17 No. 4 p.41 ~ p.53
|
|
|
Effects of Low Frequency Electroacupuncture on the Paw Edema and Phosphorylation of Spinal N-methyl-D-aspartate Receptor in the Carrageenan-injected Rat
|
|
Lee Sang-Yong
Nam Jung-Hun
Kim Bong-Hyun
Lee In-Seon
|
|
|
Abstract
|
|
|
Objectives :This study was carried out to investigate the effects of low frequency 2 Hz electroacupuncture(EA) on the paw edema and phosphorylation of spinal N-methyl-D-aspartate(NMDA) receptor.
Methods :Inflammation was induced by an intraplantar injection of 1% carrageenan into the right hind paw. Bilateral EA stimulation with 2 Hz were delivered at those acupoints corresponding to human Zusanli(S36) and Sanyinjiao(Sp6) via the needles for a total of 30min duration.
Results :1. The 2 Hz EA stimulation presented significant edema inhibition with reduced degranulation of mast cells in the paw connective tissue and this stimulation also reduced the development of both thermal and mechanical hyperalgesia. 2. The number of c-fos immunoreactive neurons was significantly increased in the superficial laminae and nucleus proprius of the dorsal horn at L4-5 spinal segment by carrageenan injection. But the immunoreactive neurons in superficial laminae of the dorsal horn markedly reduced by EA stimulation. 3. The results of Western blot showed that the level of NR1 subunit, phoshphorylated NR1 and NR2B subunits was induced by carrageenan injection, but there were no significant changes by EA stimulation. 4. In immunohistochemical analysis, the phosphorylated NR2B immunoreaction also expressed highly in the superficial laminae of spinal cord by carrageenan injection. But the phosphorylated NR2B subunit was suppressed by EA stimulation.
Conclusion :These results indicate that low frequency EA stimulation have an alleviating function against carrageenan-induced edema and hyperalgesia pain through modulating NMDA receptors and its phosphorylation.
|
|
|
KEYWORD
|
|
|
Electroacupuncture, Phosphorylation, N-methyl-D-aspartate receptor, Inflamation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|